Anti-COT (Phospho-Thr290) polyclonal Antibody detects COT only when phosphorylated at Thr290. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
-
Product Name
COT (Phospho-Thr290) Antibody
-
Catalog No
A0064
-
Storage/Stability
Stable at -20°C for at least 1 year.
-
Applications
WB, IHC, IF, ELISA
-
Clonality
Polyclonal
-
Host Species
Rabbit
-
Reactivity
Human, Mouse, Rat
-
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
-
Database Links
Gene ID: 1326, Swiss-Prot #: P41279, OMIM #: 191195, Unigene #: Hs.432453
-
Immunogen
The antiserum was produced against synthesized peptide derived from human COT around the phosphorylation site of Thr290.
-
Immunogen Range
256-305
-
Modification Sites
H: T290 M: T290 R: T290
-
MW
52 kDa
-
NCBI Gene Symbol
MAP3K8
-
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
-
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
-
Specificity
COT (Phospho-Thr290) Antibody detects endogenous levels of COT only when phosphorylated at Thr290.
-
Synonyms
C-COT, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, M3K8, MAP3K8, Mitogen-activated protein kinase kinase kinase 8, TPL-2, TPL2, Tpl2, Tumor progression locus 2, kinase Cot
-
Target Modification
Phospho
Application Images
Western blot analysis of lysates from 293 cells treated with UV 15', using COT (Phospho-Thr290) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using COT (Phospho-Thr290) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HUVEC cells, using COT (Phospho-Thr290) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-Cot (T290) Antibody
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
|
Western blot analysis of lysates from 293 cells treated with UV 15', using COT (Phospho-Thr290) Antibody. The lane on the right is blocked with the phospho peptide. |
|
Immunohistochemistry analysis of paraffin-embedded human brain, using COT (Phospho-Thr290) Antibody. The picture on the right is blocked with the phospho peptide. |
|
Immunofluorescence analysis of HUVEC cells, using COT (Phospho-Thr290) Antibody. The picture on the right is blocked with the phospho peptide. |
|
Western Blot analysis of various cells using Phospho-Cot (T290) Antibody |
|
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
Follow us on Twitter
Follow us on Linkedin
Like us on Facebook
Subscribe to our Channel