Anti-CK-1 alpha (Phospho-Tyr294) polyclonal Antibody detects CK-1 alpha only when phosphorylated at Tyr294. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
CK-1 alpha (Phospho-Tyr294) Antibody
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Catalog No
A8088
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
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Database Links
Gene ID: 1452/122011, Swiss-Prot #: P48729/Q8N752, OMIM #: 600505, Unigene #: Hs.529862/Hs.712555/Hs.512897
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Immunogen
The antiserum was produced against synthesized peptide derived from human CK-1 alpha around the phosphorylation site of Tyr294.
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Immunogen Range
260-309
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Modification Sites
H: Y294 M: Y294 R: Y294
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MW
38 kDa
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NCBI Gene Symbol
CSNK1A1
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
CK-1 alpha (Phospho-Tyr294) Antibody detects endogenous levels of CK-1 alpha only when phosphorylated at Tyr294.
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Synonyms
CK1-alpha, CKI-alpha, CSNK1A1, Casein kinase I, alpha isoform, KC1A, kinase CK1-alpha
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from HT29 cells treated with heat shock, using CK-1 alpha (Phospho-Tyr294) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using CK-1 alpha (Phospho-Tyr294) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of HBE cells using Phospho-Casein Kinase Ialpha (Y294) Antibody diluted at 1:1000
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Western blot analysis of lysates from HT29 cells treated with heat shock, using CK-1 alpha (Phospho-Tyr294) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human brain, using CK-1 alpha (Phospho-Tyr294) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western Blot analysis of HBE cells using Phospho-Casein Kinase Ialpha (Y294) Antibody diluted at 1:1000 |
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