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CDC25C (Phospho-Thr48) Fluorometric Cell-Based ELISA Kit

Catalog No : FLUO-CBP1247
  • Reactivity
    Human
  • Assay Type
    Cell-Based ELISA
  • Size
    1 Kit
  • Price
    $480.00
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The CDC25C (Phospho-Thr48) Fluorometric Cell-Based Phospho ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CDC25C (Phospho-Thr48) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated CDC25C (Phospho-Thr48) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CDC25A phosphorylation.
Details
  • Product Name
  • Catalog No
  • Detection Method
    Fluorometric
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human
  • Assay Type
    Cell-Based ELISA
  • Database Links
    Gene ID: 995, UniProt ID: P30307, OMIM #: 157680, Unigene #: Hs.656
  • Format
    Two 96-Well Plates
  • Manual (PDF)
  • NCBI Gene Symbol
    CDC25A
  • Sub Type
    Phospho
  • Synonyms
    CDC25M1, dual specificity phosphatase Cdc25C, M-phase inducer phosphatase 3, MPI1B, MPIP3
  • TargetName
    Phospho-CDC25C (Thr48)
Application Images
Image 1 Western blot analysis of extracts from RAW264.7 cells, using CDC25C (Phospho-Thr48) Antibody.
Image 2 HeLa cells were starved for 24 hrs. and stimulated with 1mM Vanadate for 1 hr.
Image 3 An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Image 4 A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Image 5 The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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