Anti-C-RAF (Phospho-Ser43) polyclonal Antibody detects C-RAF only when phosphorylated at Ser43. Validated for WB and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
C-RAF (Phospho-Ser43) Antibody
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Catalog No
A0893
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
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Database Links
Gene ID: 5894, Swiss-Prot #: P04049, OMIM #: 164760/611553/611554, Unigene #: Hs.159130
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Immunogen
The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser43.
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Immunogen Range
11-60
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Modification Sites
H: S43 M: S43 R: S43
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MW
73 kDa
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NCBI Gene Symbol
RAF1
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
C-RAF (Phospho-Ser43) Antibody detects endogenous levels of C-RAF only when phosphorylated at Ser43.
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Synonyms
C-RAF, C-Raf, CRAF, kinase Raf1, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, Raf proto-oncogene serine/threonine-protein kinase, RAF-1, Raf-1, RAF1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1
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Target Modification
Phospho
Application Images
| Image 1 |
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Western blot analysis of lysates from NIH/3T3 cells, using C-RAF (Phospho-Ser43) Antibody. The lane on the right is blocked with the phospho peptide. |
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