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C/EBP-beta (Phospho-Thr235/188) Antibody

Catalog No : A0057
  • Applications
    WB , IHC , IF , ELISA
  • Reactivity
    Human , Mouse , Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
  • Size
    50ul
  • Price
    $195.00
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Anti-C/EBP-beta (Phospho-Thr235/188) polyclonal Antibody detects C/EBP-beta only when phosphorylated at Thr235/188. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
  • Database Links
    Gene ID: 1051, Swiss-Prot #: P17676, OMIM #: 189965, Unigene #: Hs.517106/Hs.701858/Hs.712709
  • Image 1
    Immunofluorescence analysis of HepG2 cells, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is blocked with the phospho peptide.
  • Image 2
    Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human C/EBP-beta around the phosphorylation site of Thr235/188.
  • Immunogen Range
    201-250
  • Modification Sites
    H: T235 M: T188 R: T189
  • MW
    36 kDa
  • NCBI Gene Symbol
    CEBPB
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    C/EBP-beta (Phospho-Thr235/188) Antibody detects endogenous levels of C/EBP-beta only when phosphorylated at Thr235/188.
  • Synonyms
    AGP/EBP, C/EBP beta, C/EBP-related protein 2, CCAAT/enhancer binding protein beta, CEBPB, CRP2, IL-6DBP, Interleukin-6- dependent binding protein, LAP, Liver-enriched transcriptional activator, Nuclear factor NF-IL6, SF-B, SFB, Silencer factor B, TCF5, Tr
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using C/EBP-beta (Phospho-Thr235/188) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Immunofluorescence analysis of HepG2 cells, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is blocked with the phospho peptide.
Image 4 Western Blot analysis of various cells using Phospho-C/EBP beta (T235) Antibody diluted at 1:500
Image 5 Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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