Anti-C/EBP-beta (Phospho-Thr235/188) polyclonal Antibody detects C/EBP-beta only when phosphorylated at Thr235/188. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
C/EBP-beta (Phospho-Thr235/188) Antibody
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Catalog No
A0057
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, IF, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
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Database Links
Gene ID: 1051, Swiss-Prot #: P17676, OMIM #: 189965, Unigene #: Hs.517106/Hs.701858/Hs.712709
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Immunogen
The antiserum was produced against synthesized peptide derived from human C/EBP-beta around the phosphorylation site of Thr235/188.
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Immunogen Range
201-250
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Modification Sites
H: T235 M: T188 R: T189
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MW
36 kDa
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NCBI Gene Symbol
CEBPB
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
C/EBP-beta (Phospho-Thr235/188) Antibody detects endogenous levels of C/EBP-beta only when phosphorylated at Thr235/188.
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Synonyms
AGP/EBP, C/EBP beta, C/EBP-related protein 2, CCAAT/enhancer binding protein beta, CEBPB, CRP2, IL-6DBP, Interleukin-6- dependent binding protein, LAP, Liver-enriched transcriptional activator, Nuclear factor NF-IL6, SF-B, SFB, Silencer factor B, TCF5, Tr
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using C/EBP-beta (Phospho-Thr235/188) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HepG2 cells, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-C/EBP beta (T235) Antibody diluted at 1:500
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using C/EBP-beta (Phospho-Thr235/188) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is blocked with the phospho peptide. |
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Immunofluorescence analysis of HepG2 cells, using C/EBP-beta (Phospho-Thr235/188) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western Blot analysis of various cells using Phospho-C/EBP beta (T235) Antibody diluted at 1:500 |
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
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