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BTK (Phospho-Tyr223) Antibody

Catalog No : A0054
  • Applications
    WB , ELISA
  • Reactivity
    Human , Mouse , Rat
  • Application Dilutions
    WB: 1:500~1:1000 ELISA: 1:40000
  • Size
  • Price
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Anti-BTK (Phospho-Tyr223) polyclonal Antibody detects BTK only when phosphorylated at Tyr223. Validated for WB and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
  • Product Name
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
  • Clonality
  • Host Species
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 ELISA: 1:40000
  • Database Links
    Gene ID: 695, Swiss-Prot #: Q06187, OMIM #: 300300/300755/307200, Unigene #: Hs.159494
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human BTK around the phosphorylation site of Tyr223.
  • Immunogen Range
  • Modification Sites
    H: Y223 M: Y223 R: Y224
  • MW
    76 kDa
  • NCBI Gene Symbol
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    BTK (Phospho-Tyr223) Antibody detects endogenous levels of BTK only when phosphorylated at Tyr223.
  • Target Modification
Application Images
Image 1 Western blot analysis of lysates from HeLa cells treated with Serum 10% 15', using BTK (Phospho-Tyr223) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Western Blot analysis of various cells using Phospho-Btk (Y223) Antibody
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