Anti-BTK (Phospho-Tyr223) polyclonal Antibody detects BTK only when phosphorylated at Tyr223. Validated for WB and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Stable at -20°C for at least 1 year.
Human, Mouse, Rat
WB: 1:500~1:1000 ELISA: 1:40000
Gene ID: 695, Swiss-Prot #: Q06187, OMIM #: 300300/300755/307200, Unigene #: Hs.159494
The antiserum was produced against synthesized peptide derived from human BTK around the phosphorylation site of Tyr223.
H: Y223 M: Y223 R: Y224
NCBI Gene Symbol
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
BTK (Phospho-Tyr223) Antibody detects endogenous levels of BTK only when phosphorylated at Tyr223.
||Western blot analysis of lysates from HeLa cells treated with Serum 10% 15', using BTK (Phospho-Tyr223) Antibody. The lane on the right is blocked with the phospho peptide.