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Alanine Aminotransferase Assay Kit II- Colorimetric

Catalog No : EZ-0503
  • Size
    200 Assays
  • Price
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ALT catalyzes the alanine cycles and is important in amino acid metabolism. It transfers an α-amino group from alanine to α-ketoglutarate in a reversible transamination reaction to produce pyruvate and glutamate. The glutamate product from ALT catalysis can be coupled to an enzyme reaction cycle to form blue-colored product. Signal increases with increasing ALT levels and can be read with absorbance microplate reader at 575 nm or with greater sensitivity using absorbance ratio of A570nm/A610nm. 

Application Images
Image 1
Image 2 The Alanine Aminotransferase Assay Kit II- Colorimetric allows for the detection and quantification of endogenous Alanine Aminotransferase within the range of 1 mU/ml - 1000 mU/ml in cell lysates, sera, and plasma.
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