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Alanine Aminotransferase Assay Kit II- Colorimetric

Catalog No : EZ-0503
  • Size
    200 Assays
  • Price
    $295.00

ALT catalyzes the alanine cycles and is important in amino acid metabolism. It transfers an α-amino group from alanine to α-ketoglutarate in a reversible transamination reaction to produce pyruvate and glutamate. The glutamate product from ALT catalysis can be coupled to an enzyme reaction cycle to form blue-colored product. Signal increases with increasing ALT levels and can be read with absorbance microplate reader at 575 nm or with greater sensitivity using absorbance ratio of A570nm/A610nm. 

Details
  • Product Name
    Alanine Aminotransferase Assay Kit II- Colorimetric
  • Catalog No
    EZ-0503
  • Detection Method
    Colorimetric
  • Storage/Stability
    -20°, C/3 Months
  • Colorimetric Readout (nm)
    575 nm
  • Manual
    Download
  • Range Detection
    1 mU/ml - 1000 mU/ml
  • Tar Name
    Alanine Aminotransferase
Application Images
Image 1
Image 2 The Alanine Aminotransferase Assay Kit II- Colorimetric allows for the detection and quantification of endogenous Alanine Aminotransferase within the range of 1 mU/ml - 1000 mU/ml in cell lysates, sera, and plasma.