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Akt2 (Phospho-Ser474) Antibody

Catalog No : A7006
  • Applications
    WB , IHC , ELISA
  • Reactivity
    Human , Mouse , Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
  • Size
    50ul
  • Price
    $195.00
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Anti-Akt2 (Phospho-Ser474) polyclonal Antibody detects Akt2 only when phosphorylated at Ser474. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
  • Database Links
    Gene ID: 208, Swiss-Prot #: P31751, OMIM #: 164731, Unigene #: Hs.631535
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Akt2 around the phosphorylation site of Ser474.
  • Immunogen Range
    432-481
  • Modification Sites
    H: S474 M: S474 R: S474
  • MW
    55 kDa
  • NCBI Gene Symbol
    AKT2
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    Akt2 (Phospho-Ser474) Antibody detects endogenous levels of Akt2 only when phosphorylated at Ser474.
  • Synonyms
    kinase Akt2, PKB beta, Protein kinase Akt-2, Protein kinase B, beta, RAC-beta serine/threonine protein kinase, RAC-PK-beta, v-akt murine thymoma viral oncogene homolog 2
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using Akt2 (Phospho-Ser474) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Akt2 (Phospho-Ser474) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western Blot analysis of various cells using Phospho-Akt2 (S474) Antibody diluted at 1:500
Image 4 Western Blot analysis of 293 cells using Phospho-Akt2 (S474) Antibody diluted at 1:500
References
This product has been referenced in:
  • "Maruf Hasan1,4,5, Bernhard Neumann1,4, Steffen Haupeltshofer2,4, Sarah Stahlke2, Massimo Claud "Activation of TGF-β-induced non-Smad signaling pathways during Th17 differentiation" Immunology and Cell Biology (2015) 93, 662–672 Primary Antibody Read more >
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