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Akt1 S1 (Phospho-Thr246) Antibody

Catalog No : A0453
  • Applications
    WB, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    100ul
  • Price
    $295.00
Anti-Akt1 S1 (Phospho-Thr246) polyclonal Antibody detects Akt1 S1 only when phosphorylated at Thr246. Validated for WB and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    Akt1 S1 (Phospho-Thr246) Antibody
  • Catalog No
    A0453
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 ELISA: 1:10000
  • Database Links
    Gene ID: 84335, Swiss-Prot #: Q96B36, OMIM #: 610221, Unigene #: Hs.515542
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Akt1 S1 around the phosphorylation site of Thr246.
  • Immunogen Range
    207-256
  • Modification Sites
    H: T246 M: T247 R: T262
  • MW
    27 kDa
  • NCBI Gene Symbol
    AKT1S1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    Akt1 S1 (Phospho-Thr246) Antibody detects endogenous levels of Akt1 S1 only when phosphorylated at Thr246.
  • Synonyms
    Akt1 substrate 1 (proline-rich), AKTS1, Lobe, MGC2865, PRAS40, proline-rich Akt substrate 40 kDa
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from HepG2 cells treated with PDGF 50ng/ml 30', using Akt1 S1 (Phospho-Thr246) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HepG2 cells treated with PDGF 50ng/ml 30', using Akt1 S1 (Phospho-Thr246) Antibody. The lane on the right is blocked with the phospho peptide.
References
This product has been referenced in:
  • Avgi Tsolou,1 Maria Liousia,1 Dimitra Kalamida,1 Stamatia Pouliliou,1 Alexandra Giatromanolaki,2 and "Inhibition of IKK-NFκB pathway sensitizes lung cancer cell lines to radiation " NCBIPMC Membranes were probed with the appropriate primary antibodies: "doi: 10.20892/j.issn.2095-3941.2017.0049 PMCID: PMC5570606 PMID: 28884046" Read more >