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ATP Assay Kit - Luminometric

Catalog No : EZ-0537
  • Size
    100 Assays
  • Price
This ultrasensitive assay allows for determination of cell proliferation and cytotoxicity in mammalian cells. ATP has been found to be essential in light production in bioluminescence of firefly luciferase, a monomeric 61 kD enzyme that catalyzes a two-step oxidation of luciferin to yield light at 560 nm. The first step consists of protein activation by ATP to produce an intermediate that is a reactive mixed anhydride. This intermediate reacts with oxygen in a second step to create a transient dioxetane, which is quickly broken down into the oxidized products of oxyluciferin and carbon dioxide, along with the burst of light. With ATP as a limiting component, this light intensity is proportional to ATP concentration and can be monitored at 560 nm with a luminometer or other luminescence instruments. 
  • Product Name
    ATP Assay Kit - Luminometric
  • Catalog No
  • Detection Method
  • Storage/Stability
    -20°, C/3 Months
  • Manual
  • Range Detection
    1 nM - 1000 nM
  • Tar Name
Application Images
Image 1 The ATP Assay Kit - Luminometric allows for the detection and quantification of endogenous ATP within the range of 1 to 1000 nM in cell lysates, sera, and plasma.
Image 2