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ATP Assay Kit II - Luminometric

Catalog No : EZ-0538
  • Size
    200 Assays
  • Price
    $145.00
This assay allows for determination of cell proliferation and cytotoxicity in mammalian cells. The first step consists of protein activation by ATP to produce an intermediate that is a reactive mixed anhydride. This intermediate reacts with oxygen in a second step to create a transient dioxetane, which is quickly broken down into the oxidized products of oxyluciferin and carbon dioxide, along with the burst of light. With ATP as a limiting component, this light intensity is proportional to ATP concentration and can be monitored at 560 nm with a luminometer or other luminescence instruments. This kit provides a steady luminescence signal that lasts for a few hours, making it convenient for use with luminometers that are not equipped with liquid handling capacity. Although not as sensitive as “Bright Glow” assay, the stable luminescence signal allows for unique uses such as a rapid diagnosis applications in rapid food safety inspections and hand-held luminomenters. 
Details
  • Product Name
    ATP Assay Kit II - Luminometric
  • Catalog No
    EZ-0538
  • Detection Method
    Luminometric
  • Storage/Stability
    -20°, C/3 Months
  • Manual
    Download
  • Range Detection
    1 nM - 10000 nM
  • Tar Name
    ATP
Application Images
The ATP Assay Kit II - Luminometric allows for the detection and quantification of endogenous ATP within the range of 1 to 10000 nM in cell lysates, sera, and plasma.
The ATP Assay Kit II - Luminometric allows for the detection and quantification of endogenous ATP within the range of 1 to 10000 nM in cell lysates, sera, and plasma.