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ATF2 (Phospho-Thr71 or 53) Antibody

Catalog No : A7015
  • Applications
    WB , IHC , IP , ELISA
  • Reactivity
    Human , Mouse , Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
  • Size
    50ul
  • Price
    $195.00
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Anti-ATF2 (Phospho-Thr71 or 53) polyclonal Antibody detects ATF2 only when phosphorylated at Thr71 or 53. Validated for WB IHC IP ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IP, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
  • Database Links
    Gene ID: 1386, Swiss-Prot #: P15336, OMIM #: 123811, Unigene #: Hs.592510
  • Image 1
    Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ATF2 (Phospho-Thr71 or 53) Antibody. The picture on the right is blocked with the phospho peptide.
  • Image 2
    Western blot analysis of lysates from HeLa cells treated with UV, using ATF2 (Phospho-Thr71 or 53) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human ATF2 around the phosphorylation site of Thr71 or 53.
  • Immunogen Range
    38-87
  • Modification Sites
    H: T71 M: T53 R: T53
  • MW
    52 kDa
  • NCBI Gene Symbol
    ATF2
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    ATF2 (Phospho-Thr71 or 53) Antibody detects endogenous levels of ATF2 only when phosphorylated at Thr71 or 53.
  • Synonyms
    Activating 2, ATF2, cAMP response element binding protein CRE- BP1, CREB2, CREBP1, Cyclic-AMP-dependent ATF-2, HB16, MXBP protein
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from HeLa cells treated with UV, using ATF2 (Phospho-Thr71 or 53) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ATF2 (Phospho-Thr71 or 53) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western Blot analysis of various cells using Phospho-ATF-2 (T71) Antibody diluted at 1:1000
Image 4 Western Blot analysis of HuvEc cells using Phospho-ATF-2 (T71) Antibody diluted at 1:1000
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