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AMPKalpha (Phospho-Thr172) Fluorometric Cell-Based ELISA Kit

Catalog No : FLUO-CBP1003
  • Reactivity
    Human , Mouse , Rat
  • Assay Type
    Cell-Based ELISA
  • Size
    3 Kits
  • Price
    Inquire
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The AMPKalpha (Phospho-Thr172) Fluorometric Cell-Based Phospho ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AMPK? (Phospho-Thr172) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated AMPK? (Phospho-Thr172) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on AAPK1/AAPK2 phosphorylation.
Details
  • Product Name
  • Catalog No
  • Detection Method
    Fluorometric
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    Cell-Based ELISA
  • Database Links
    Gene ID: 5562/5563, UniProt ID: Q13131/P54646, OMIM #: 602739/600497, Unigene #: Hs.43322/Hs.437039
  • Format
    Two 96-Well Plates
  • Manual (PDF)
    <a href='image/catalog/assbiotech/FLUO-CBP1003-2018.pdf'>Download</a>
  • NCBI Gene Symbol
    AAPK1/AAPK2
  • Sub Type
    Phospho
  • TargetName
    Phospho-AMPKα (Thr172)
Application Images
Image 1 Western blot analysis of extracts from COS7 cells, using AMPK alpha (Phospho-Thr172) Antibody.
Image 2 HeLa cells starved for 24 hrs. and stimulated with LPS 100ng/ml 2 hrs.
Image 3 An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Image 4 A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Image 5 The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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