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AMPK alpha 1/2 (Phospho-Thr183/172) Antibody

Catalog No : A0003
  • Applications
    WB,
  • Applications
    IHC,
  • Applications
    ELISA
  • Reactivity
    Human,
  • Reactivity
    Mouse,
  • Reactivity
    Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
  • Size
    50ul
  • Price
    $195.00
Anti-AMPK alpha (Phospho-Thr172) polyclonal Antibody detects AMPK alpha only when phosphorylated at Thr172. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    AMPK alpha 1/2 (Phospho-Thr183/172) Antibody
  • Catalog No
    A0003
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
  • Database Links
    Gene ID: 5562/5563, Swiss-Prot #: Q13131/P54646, OMIM #: 602739/600497, Unigene #: Hs.43322/Hs.437039
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human AMPK alpha 1/2 around the phosphorylation site of Thr183/172.
  • Immunogen Range
    140-189
  • Modification Sites
    H: T183/172 M: T183/172 R: T183/172
  • MW
    62 kDa
  • NCBI Gene Symbol
    PRKAA1, PRKAA2
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    AMPK alpha 1/2 (Phospho-Thr183/172) Antibody detects endogenous levels of AMPK alpha 1/2 only when phosphorylated at Thr183/172.
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from COS7 cells, using AMPK alpha (Phospho-Thr172) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AMPK alpha (Phospho-Thr172) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western Blot analysis of mouse-lung cells using primary antibody diluted at 1:1000(4°C ON). Secondary antibody Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour).
Image 4 Western Blot analysis of various cells using Phospho-AMPKalpha1/2 (T183/172) Antibody diluted at 1:500
Image 5 Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Image 6 Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Image 7 Immunofluorescence analysis of rat-heart tissue. AMPKalpha1/2 (phospho Thr183/172) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B