Call (408) 747-0185 or Contact us .

ADD1 (Phospho-Ser726) Antibody

Catalog No : A0002
  • Applications
    WB , IHC , IF , ELISA
  • Reactivity
    Human , Mouse , Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
  • Size
    50ul
  • Price
    $195.00
Add to Cart
Order by PO? Email to Order@assaybiotech.com
Technical Questions? Email to Tech@assaybiotech. com
Anti-ADD1 (Phospho-Ser726) polyclonal Antibody detects ADD1 only when phosphorylated at Ser726. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
  • Database Links
    Gene ID: 118/119, Swiss-Prot #: P35611/P35612, OMIM #: 102680/102681, Unigene #: Hs.183706/Hs.701490/Hs.188528
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human ADD1 around the phosphorylation site of Ser726.
  • Immunogen Range
    688-737
  • Modification Sites
    H: S726/S713 M: S724/S712 R: S724/S712
  • MW
    80 kDa
  • NCBI Gene Symbol
    ADD1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    ADD1 (Phospho-Ser726) Antibody detects endogenous levels of ADD1 only when phosphorylated at Ser726.
  • Synonyms
    Alpha-adducin, Erythrocyte adducin subunit alpha , ADD1 , ADDA
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from HeLa cells treated with Forskolin 40nM 30', using ADD1 (Phospho-Ser726) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Immunofluorescence analysis of HeLa cells, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is blocked with the phospho peptide.
Image 4 Western Blot analysis of various cells using Phospho-Adducin alpha/beta (S726/713) Antibody diluted at 1:1000
Image 5 Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Get resources and offers direct to your inbox Sign up