The 4E-BP1 Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect 4E-BP1 protein expressino profile in cells. The kit can be used for measuring the relative amounts of 4E-BP1 in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on 4E-BP1.
Details
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Product Name
4E-BP1 Colorimetric Cell-Based ELISA Kit
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Catalog No
CB5008
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Detection Method
Colorimetric 450 nm
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Dynamic Range
> 5000 Cells
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Storage/Stability
4°C/6 Months
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Reactivity
Human, Mouse, Rat
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Assay Type
Cell-Based
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Database Links
Gene ID: 1978, UniProt ID: Q13541, OMIM #: 602223, Unigene #: Hs.411641
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Format
96-Well Microplate
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Manual (PDF)
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NCBI Gene Symbol
EIF4EBP1
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Sub Type
None
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Synonyms
4EBP1, EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Insulin-stimulated EIF-4E binding protein PHAS-I, P/OKCL.6, PHAS-1, PHAS-I
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TargetName
4E-BP1
Application Images
Western blot analysis of extracts from Jurkat cells, treated with Insulin 0.01U/ml 15', using 4E-BP1 (Ab-64) Antibody.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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Western blot analysis of extracts from Jurkat cells, treated with Insulin 0.01U/ml 15', using 4E-BP1 (Ab-64) Antibody. |
|
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles. |
|
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits. |
|
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity. |