Anti-14-3-3 zeta (Phospho-Ser58) polyclonal Antibody detects 14-3-3 zeta only when phosphorylated at Ser58. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
-
Product Name
14-3-3 zeta (Phospho-Ser58) Antibody
-
Catalog No
A0001
-
Storage/Stability
Stable at -20°C for at least 1 year.
-
Applications
WB, IHC, IF, ELISA
-
Clonality
Polyclonal
-
Host Species
Rabbit
-
Reactivity
Human, Mouse, Rat
-
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
-
Database Links
Gene ID: 7534, Swiss-Prot #: P63104, OMIM #: 601288, Unigene #: Hs.492407/Hs.594673
-
Immunogen
The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58.
-
Immunogen Range
24-73
-
Modification Sites
H: S58 M: S58 R: S58
-
MW
27 kDa
-
NCBI Gene Symbol
YWHAZ
-
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
-
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
-
Specificity
14-3-3 zeta (Phospho-Ser58) Antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at Ser58.
-
Synonyms
14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ
-
Target Modification
Phospho
Application Images
Western blot analysis of lysates from NIH/3T3 cells treated with UV 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-14-3-3 zeta (S58) Antibody diluted at 1:2000
Western Blot analysis of Jurkat cells using Phospho-14-3-3 zeta (S58) Antibody diluted at 1:2000
|
Western blot analysis of lysates from NIH/3T3 cells treated with UV 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The lane on the right is blocked with the phospho peptide. |
|
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide. |
|
Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide. |
|
Western Blot analysis of various cells using Phospho-14-3-3 zeta (S58) Antibody diluted at 1:2000 |
|
Western Blot analysis of Jurkat cells using Phospho-14-3-3 zeta (S58) Antibody diluted at 1:2000 |
Follow us on Twitter
Follow us on Linkedin
Like us on Facebook
Subscribe to our Channel