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> Products > Antibodies > 14-3-3 zeta (Phospho-Ser58) Antibody

14-3-3 zeta (Phospho-Ser58) Antibody

Datasheet SDS
Catalog No : A0001
  • Applications
    WB, IHC, IF, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    100ul
  • Price
    $295.00
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Anti-14-3-3 zeta (Phospho-Ser58) polyclonal Antibody detects 14-3-3 zeta only when phosphorylated at Ser58. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    14-3-3 zeta (Phospho-Ser58) Antibody
  • Catalog No
    A0001
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
  • Database Links
    Gene ID: 7534, Swiss-Prot #: P63104, OMIM #: 601288, Unigene #: Hs.492407/Hs.594673
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58.
  • Immunogen Range
    24-73
  • Modification Sites
    H: S58 M: S58 R: S58
  • MW
    27 kDa
  • NCBI Gene Symbol
    YWHAZ
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    14-3-3 zeta (Phospho-Ser58) Antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at Ser58.
  • Synonyms
    14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from NIH/3T3 cells treated with UV 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.
Image 4 Western Blot analysis of various cells using Phospho-14-3-3 zeta (S58) Antibody diluted at 1:2000
Image 5 Western Blot analysis of Jurkat cells using Phospho-14-3-3 zeta (S58) Antibody diluted at 1:2000
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