The 14-3-3 zeta Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect 14-3-3 zeta protein expressino profile in cells. The kit can be used for measuring the relative amounts of 14-3-3 zeta in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on 14-3-3 zeta.
Details
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Product Name
14-3-3 zeta Colorimetric Cell-Based ELISA Kit
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Catalog No
CB5002
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Detection Method
Colorimetric 450 nm
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Dynamic Range
> 5000 Cells
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Storage/Stability
4°C/6 Months
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Reactivity
Human, Mouse, Rat
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Assay Type
Cell-Based
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Database Links
Gene ID: 7534, UniProt ID: P63104, OMIM #: 601288, Unigene #: Hs.492407/Hs.594673
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Format
96-Well Microplate
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Manual (PDF)
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NCBI Gene Symbol
YWHAZ
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Sub Type
None
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Synonyms
14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ
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TargetName
14-3-3 zeta
Application Images
Western blot analysis of extracts from 293 cells, treated with Forskolin 40nM 30', using 14-3-3 zeta (Ab-58) Antibody.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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Western blot analysis of extracts from 293 cells, treated with Forskolin 40nM 30', using 14-3-3 zeta (Ab-58) Antibody. |
|
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles. |
|
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits. |
|
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity. |
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