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IkappaB-epsilon (Phospho-Ser22) Antibody

Catalog No : A0015
  • Applications
    WB , IHC , IF , ELISA
  • Reactivity
    Human , Mouse , Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
  • Size
    50ul
  • Price
    $195.00
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Anti-IkappaB-epsilon (Phospho-Ser22) polyclonal Antibody detects IkappaB-epsilon only when phosphorylated at Ser22. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
  • Database Links
    Gene ID: 4794, Swiss-Prot #: O00221, OMIM #: 604548, Unigene #: Hs.458276
  • Image 1
    Immunofluorescence analysis of HeLa cells, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.
  • Image 2
    Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human IkappaB-epsilon around the phosphorylation site of Ser22.
  • Immunogen Range
    131-180
  • Modification Sites
    H: S22 M: S22 R: S22
  • MW
    38 kDa
  • NCBI Gene Symbol
    NFKBIE
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    IkappaB-epsilon (Phospho-Ser22) Antibody detects endogenous levels of IkappaB-epsilon only when phosphorylated at Ser22.
  • Synonyms
    I-kappa-B-epsilon, IKBE, IkappaBepsilon, NF-kappa-BIE, NF-kappaB inhibitor epsilon, NFKBIE
  • Target Modification
    Phospho
Application Images
Image 1 Immunofluorescence analysis of HeLa cells, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western blot analysis of lysates from Jurkat cells treated with TNF-a 20ng/ml 30', using IkappaB-epsilon (Phospho-Ser22) Antibody. The lane on the right is blocked with the phospho peptide.
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