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EGFR (Phospho-Thr678) Antibody

Catalog No : A0008
  • Applications
    WB, IF, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000
  • Size
    50ul
  • Price
    $195.00
Details
  • Product Name
    EGFR (Phospho-Thr678) Antibody
  • Catalog No
    A0008
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000
  • Database Links
    Gene ID: 1956, Swiss-Prot #: P00533, OMIM #: 131550/211980, Unigene #: Hs.488293
  • Image 1
    Immunofluorescence analysis of HUVEC cells, using EGFR (Phospho-Thr678) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Thr678.
  • Immunogen Range
    651-700
  • Modification Sites
    H: T678 M: T680 R: T679
  • MW
    134 kDa
  • NCBI Gene Symbol
    EGFR
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    EGFR (Phospho-Thr678) Antibody detects endogenous levels of EGFR only when phosphorylated at Thr678.
  • Synonyms
    Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 , EGFR , ERBB1
  • Target Modification
    Phospho
Application Images
Image 1 Immunofluorescence analysis of HUVEC cells, using EGFR (Phospho-Thr678) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using EGFR (Phospho-Thr678) Antibody. The lane on the right is blocked with the phospho peptide.
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