anti-14-3-3 zeta (Phospho-Ser58) polyclonal antibody detects 14-3-3 zeta only when phosphorylated at Ser58. Validated for WB, IHC, IF and ELISA. Tested on and reactive for Human, Mouse, and Rabbit. Original Antibody Manufacturer.
The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58.
H: S58 M: S58 R: S58
NCBI Gene Symbol
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
14-3-3 zeta (Phospho-Ser58) Antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at Ser58.
14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ
Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from NIH/3T3 cells treated with UV 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The lane on the right is blocked with the phospho peptide.