Existing methods to detect NADP/NADPH require exhibit low sensitivity and high interference. These are performed using expensive quartz microplates and in the UV range. In contrast, this assay utilizes a sensitive, one-step process with enzymes specific for NADP/NADPH, resulting in high detection sensitivity due to an enzyme cycling reaction. A longer wavelength range reduces the interference from biological samples, resulting in low background fluorescence signals. The plate can be read by an absorbance microplate reader at 575 nm, or with greater sensitivity using absorbance ratio of A570nm/A605nm. Purification of the NAD/NADH sample mix is not needed prior to reading.
|Image 1||The NADP/NADPH Assay Kit - Colorimetric allows for the detection and quantification of endogenous NADP/NADPH within the range of 0.003 to 10 µM in cell lysates, sera, and plasma.|