also called Ovarian carcinoma antigen CA125; Ovarian cancer-related tumor
marker CA125, is a monoclonal antibody that reacts with epithelioid
mesothelioma, clear cell adenocarcinoma of the bladder, ovarian epithelioid
malignancies, cervical papillary serous carcinoma, and endometrium
adenocarcinoma. The antigen is resistant to formalin treatment allowing it to
detect potential ovarian cancer with the help of immunohistochemistry.
Usually, ovarian cancer is monitored using the serum assay of the protein.
The antibody of CA-125 also interacts with seminal vesicle carcinoma
antigens. The antigen is retreived by the Heat-induced epitope retrieval
(HIER) system using the TRIS-EDTA buffer of pH - 8.0.