This assay can measure SOD with a highly sensitive colorimetric method. Xanthine is converted to superoxide radical ions (O2-), uric acid, and hydrogen peroxide by xanthine oxidase (XO). The superoxide is coupled to an enzyme reaction to generate a blue-colored product with absorbance at 560 nm when read by an absorbance microplate reader. SOD competes with this reaction for the superoxide substrate, reducing the signal. Thus, low signal at 560 nm correlates with high levels of SOD activity.
|Image 1||The Superoxide Dismutase Assay Kit - Colorimetric allows for the detection and quantification of endogenous Superoxide Dismutase within the range of 0.03 to 100 U/ml in cell lysates, sera, and plasma.|